某些敏化剂通过鲁米诺的光氧化作用产生的光动力作用。

Photodynamic action of some sensitizers by photooxidation of luminol.

作者信息

Wierrani Franz, Kubin Andreas, Loew Hans Günter, Henry Michael, Spängler Babette, Bodner Klaus, Grünberger Werner, Ebermann Robert, Alth Gerhart

机构信息

Viennese Society for Photophysical Diagnosis and Photodynamic Therapy, Juchgasse 25, 1030 Vienna, Austria.

出版信息

Naturwissenschaften. 2002 Oct;89(10):466-9. doi: 10.1007/s00114-002-0357-4. Epub 2002 Sep 6.

DOI:10.1007/s00114-002-0357-4

PMID:12384722

Abstract

We report the development of a novel simple experimental method which allows the comparison of new photosensitizers based on their production of reactive oxygen species. A high-performance liquid chromatography (HPLC) assay permits the monitoring of several substances (sensitizer, reactant and oxidized end product) simultaneously on a single chromatogram. Photoreactions were monitored throughout their course by the HPLC assay surveying the sensitizers' efficiency of singlet oxygen production by the oxidative decomposition of luminol. Several photosensitizers were tested: Rose Bengal, Methylene Blue, Protoporphyrin IX, Photosan III, Photofrin, Hypericin and Pseudohypericin. Additionally, photoreactions were monitored by a standard pO(2) detection system. The measurements of the two detection methods were strongly correlated. Rose Bengal proved to be the most efficient photosensitizer, clearly decreasing the luminol concentration and causing a corresponding increase in aminophthalic acid. Our experiments show that when factors necessary for photochemical reactions are absent or are blocked (antioxidants), no reaction can be detected.

摘要

我们报告了一种新型简单实验方法的开发，该方法可根据新型光敏剂产生活性氧的情况对其进行比较。高效液相色谱（HPLC）测定法能够在一张色谱图上同时监测几种物质（敏化剂、反应物和氧化终产物）。通过HPLC测定法监测整个光反应过程，该方法通过鲁米诺的氧化分解来检测敏化剂产生单线态氧的效率。测试了几种光敏剂：孟加拉玫瑰红、亚甲蓝、原卟啉IX、光森三号、卟吩姆钠、金丝桃素和假金丝桃素。此外，通过标准的pO(2)检测系统监测光反应。两种检测方法的测量结果高度相关。孟加拉玫瑰红被证明是最有效的光敏剂，它能明显降低鲁米诺浓度，并导致邻苯二甲酸氨基相应增加。我们的实验表明，当光化学反应所需的因素不存在或被阻断（抗氧化剂）时，无法检测到反应。