Feng Huyuan, An Lizhe, Tan Lingling, Hou Zongdong, Wang Xunling
School of Life Sciences, Key State Laboratory of Arid Agroecology, Lanzhou University, Lanzhou 730000.
Ying Yong Sheng Tai Xue Bao. 2002 Jul;13(7):814-8.
In order to determine the response of pollen to UV-B irradiation and the cumulative effects of UV-B exposure time on pollen germination and tube growth of plants, 19 species were investigated in vitro by exposing their pollen grains to two levels of enhanced ultravioet-B(UV-BBE) (280-320 nm, 350 and 500 mW/m2 biologically effective UV-B radiation), simulating 8% and 21% stratospheric ozone depletion in Lanzhou, China (36.04 degrees N, 1550 m), and to no UV-B (control group). Compared with the control, enhanced UV-B radiation significantly inhibited pollen germination and tube growth in most species. Higher UV-B flux rate caused greater inhibitory effect than lower UV-B radiation level. Several test plants exhibited insensitivity, even stimulation of pollen germination and tube growth to UV-B. The reduction in pollen germination rate and tube growth increased with exposure time, which indicated a cumulative effect of UV-B radiation. It is concluded that changes in pollen susceptibility to UV-B would have a serious ecological consequence under natural conditions.
为了确定花粉对UV-B辐射的响应以及UV-B暴露时间对植物花粉萌发和花粉管生长的累积影响，通过将19种植物的花粉粒暴露于两种增强的紫外线B（UV-BBE）水平（280-320纳米，350和500毫瓦/平方米生物有效UV-B辐射）来进行体外研究，这两种水平模拟了中国兰州（北纬36.04度，海拔1550米）平流层臭氧分别减少8%和21%的情况，并设置了无UV-B辐射的对照组。与对照组相比，增强的UV-B辐射显著抑制了大多数物种的花粉萌发和花粉管生长。较高的UV-B通量率比较低的UV-B辐射水平产生了更大的抑制作用。几种受试植物对UV-B表现出不敏感，甚至花粉萌发和花粉管生长受到刺激。花粉萌发率和花粉管生长的降低随着暴露时间的增加而增加，这表明UV-B辐射具有累积效应。得出的结论是，在自然条件下，花粉对UV-B敏感性的变化将产生严重的生态后果。
