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[紫杉醇合成阶段与非紫杉醇合成阶段中国红豆杉细胞之间的基因表达差异]

[Differences in gene expression between Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase].

作者信息

Hu Guo-Bin, Mei Xing-Guo, Gong Wei, Ke Tie

机构信息

School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.

出版信息

Sheng Wu Gong Cheng Xue Bao. 2002 Jul;18(4):512-5.

Abstract

In plant, evocation of secondary metabolism is associated with complex biochemical and molecular events that are regulated by developmental and environmental factors. In order to get more information about Taxol biosynthesis, comparison of mRNA populations from Taxus chinensis cells during Taxol-synthesis phase and those during non-Taxol-synthesis phase were performed by mRNA differential display. The results suggested that genes specifically expressed in the Taxol-synthesis phase might be involved in Taxol biosynthesis.

摘要

在植物中,次生代谢的激发与复杂的生化和分子事件相关联,这些事件受发育和环境因素调控。为了获取更多关于紫杉醇生物合成的信息,通过mRNA差异显示技术,对中国红豆杉细胞在紫杉醇合成阶段和非紫杉醇合成阶段的mRNA群体进行了比较。结果表明,在紫杉醇合成阶段特异性表达的基因可能参与了紫杉醇的生物合成。

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