College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036.
Biotechnol Prog. 2011 Sep-Oct;27(5):1415-20. doi: 10.1002/btpr.660. Epub 2011 Jul 15.
Exposure to ozone induced a rapid increase in the levels of the phytohormone abscisic acid (ABA) and sequentially followed by the enhancement of Taxol production in suspension cell cultures of Taxus chinensis. The observed increases in ABA and Taxol were dependent on the concentration of ozone applied to T. chinensis cell cultures. To examine the role of ABA in ozone-induced Taxol production, we pretreated the cells with ABA biosynthesis inhibitor fluridone to abolish ozone-triggered ABA generation and assayed the effect of fluridone on ozone-induced Taxol production. The results showed that pretreatment of the cells with fluridone not only suppressed the ozone-triggered ABA generation but also blocked the ozone-induced Taxol production. Moreover, our data indicate that the effect of ABA on Taxol production of T. chinensis cell cultures is dose-dependent. Interestingly, the suppression of fluridone on ozone-induced Taxol production was reversed by exogenous application of low dose of ABA, although treatment of low dose ABA alone had no effect on Taxol production of the cells. Together, the data indicated that ozone was an efficient elicitor for improving Taxol production of plant cell cultures. Furthermore, we demonstrated that ABA played critical roles in ozone-induced Taxol production of T. chinensis suspension cell cultures.
臭氧暴露会迅速增加植物激素脱落酸（ABA）的水平，随后顺次增强悬浮细胞培养的 Taxus chinensis 中的紫杉醇产量。观察到的 ABA 和紫杉醇的增加取决于应用于 T. chinensis 细胞培养物的臭氧浓度。为了研究 ABA 在臭氧诱导的 Taxol 生产中的作用，我们用 ABA 生物合成抑制剂氟啶酮预处理细胞以消除臭氧引发的 ABA 生成，并测定氟啶酮对臭氧诱导的 Taxol 生产的影响。结果表明，细胞用氟啶酮预处理不仅抑制了臭氧引发的 ABA 生成，还阻断了臭氧诱导的 Taxol 生成。此外，我们的数据表明，ABA 对 T. chinensis 细胞培养物中 Taxol 生产的影响是剂量依赖性的。有趣的是，尽管单独使用低剂量 ABA 处理对细胞 Taxol 生产没有影响，但外源施用低剂量 ABA 可逆转氟啶酮对臭氧诱导的 Taxol 生产的抑制作用。总之，这些数据表明臭氧是提高植物细胞培养物中 Taxol 产量的有效诱导剂。此外，我们证明 ABA 在臭氧诱导的 T. chinensis 悬浮细胞培养物中 Taxol 生产中起着关键作用。
