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A novel single-stranded DNA expression vector.

作者信息

Chen Yin

机构信息

CytoGenix, Inc., 9881 S. Wilcrest Drive, Houston, TX 77099, USA.

出版信息

Expert Opin Biol Ther. 2002 Oct;2(7):735-40. doi: 10.1517/14712598.2.7.735.

DOI:10.1517/14712598.2.7.735
PMID:12387672
Abstract

A novel expression vector system has been developed that can intracellularly generate any single-stranded DNA (ssDNA) molecule, such as a triplex-forming oligonucleotide (TFO), antisense oligodeoxynucleotide (ODN) and DNA enzyme, by our laboratory at CytoGenix, Inc. 'Proof of concept' studies from our laboratory as well as our collaborators' indicate that this ssDNA expression vector system is capable of producing, intracellularly, antisense ODNs, DNA enzymes or TFOs for the purpose of downregulating gene expression or inducing targeted genome modification. This technology provides new research tools and has potential applications in gene target validation and drug development.

摘要

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