Li Zhong-Qi, Shi Zhan-Jun, Hao Wen-Bo, L Hai
Department of Orthopedics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Mar;22(3):212-4.
To establish an in vivo biopanning model of phage display peptide library in the blood vessels contained in surgically removed human osteosarcoma.
In 28 patients with osteosarcoma, digital subtraction angiography (DSA) of the involved limb was performed preoperatively to understand the approximate status of the arteries in the tumors. The tumors were then surgically removed and carefully trimmed, perfused via a simulated Langendorff perfusion apparatus with the indexes as the pH value, temperature and O2 partial pressure monitored in the blood vessels. A 12-meres phage display peptide library was biopanned to isolate peptides capable of homing specifically to the excised osteosarcoma.
In vivo biopanning models were successfully established in all the excised tumors, which could be directly used in perfusion experiment and the indexes monitored in the blood vessels in the tumors were comparable to those of living tissues. Some high-affinity peptides specific to the blood vessels in osteosarcoma were obtained with the motif of RLTR.
In vivo biopanning model simulating the Langendorff perfusion apparatus can be easily established which is instrumental for the application of phage display technology in human living tissues and may facilitate the study of targeted chemotherapy of osteosarcoma.
建立人骨肉瘤手术切除标本血管内噬菌体展示肽库的体内淘选模型。
28例骨肉瘤患者术前对患侧肢体行数字减影血管造影（DSA），了解肿瘤内动脉大致情况。然后手术切除肿瘤并仔细修整，通过模拟Langendorff灌注装置进行灌注，监测血管内的pH值、温度和氧分压等指标。用12肽噬菌体展示肽库进行体内淘选，以分离能特异性归巢至切除的骨肉瘤的肽段。
所有切除肿瘤均成功建立体内淘选模型，可直接用于灌注实验，肿瘤血管内监测的指标与活体组织相当。获得了一些以RLTR为基序的骨肉瘤血管特异性高亲和力肽段。
可轻松建立模拟Langendorff灌注装置的体内淘选模型，有助于噬菌体展示技术在人体组织中的应用，可能促进骨肉瘤靶向化疗的研究。
