Li Xi-Ping, Qian Xin-Hua, Liu Zhi-Jie
Department of Pediatrics, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Jan;22(1):51-3.
Nucleated cells were isolated from peripheral blood of healthy normal adults, dissolved and centrifuged to acquire the supernatant for RNA and the deposit for DNA extraction. Hemoglobin was extracted from the deposit formed by the red blood cells. Property assessment of the extracts indicated high quality products, demonstrating that this method is highly efficient and operable in DNA, RNA and hemoglobin extraction from the same blood sample of limited volume.
从健康正常成年人的外周血中分离出有核细胞，溶解并离心，以获取用于RNA提取的上清液和用于DNA提取的沉淀物。从红细胞形成的沉淀物中提取血红蛋白。提取物的性质评估表明产品质量高，这表明该方法在从有限体积的同一血样中提取DNA、RNA和血红蛋白方面具有高效性和可操作性。
