Gómez-Román Victor Raul, Cao Chuanhai, Bai Yun, Santamaría Hugo, Acero Gonzalo, Manoutcharian Karen, Weiner David B, Ugen Kenneth E, Gevorkian Goar
Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, DF.
J Acquir Immune Defic Syndr. 2002 Oct 1;31(2):147-53. doi: 10.1097/00126334-200210010-00004.
Antibody-dependent cellular cytotoxicity (ADCC) is a host defense mechanism in which Fc receptor-bearing effector cells in combination with antigen-specific antibodies recognize and kill antigen-expressing target cells. The authors previously described a murine monoclonal antibody (MAb-ID6) that mediated ADCC activity against HIV-infected cells. It was demonstrated that the specificity of MAb-ID6 maps to the first 204 amino acids of gp120; however, the exact epitope was not identified. In the present work, by screening phage display libraries with MAb-ID6, the authors have mapped the corresponding epitope to amino acids 86-100 (HIV-1 gp120 sequence). This epitope lies within the C1 region of gp120 and is highly conserved among all subtypes and circulating recombinant forms of HIV-1. Thus, these phage mimotopes of C1 may serve as components of a vaccine for the induction of gp120-specific antibodies mimicking MAb-ID6.
抗体依赖性细胞毒性（ADCC）是一种宿主防御机制，其中携带Fc受体的效应细胞与抗原特异性抗体结合，识别并杀死表达抗原的靶细胞。作者之前描述了一种介导针对HIV感染细胞的ADCC活性的鼠单克隆抗体（MAb-ID6）。已证明MAb-ID6的特异性定位于gp120的前204个氨基酸；然而，确切的表位尚未确定。在本研究中，通过用MAb-ID6筛选噬菌体展示文库，作者已将相应表位定位于氨基酸86 - 100（HIV-1 gp120序列）。该表位位于gp120的C1区域内，在HIV-1的所有亚型和循环重组形式中高度保守。因此，这些C1的噬菌体模拟表位可作为疫苗的组成部分，用于诱导模仿MAb-ID6的gp120特异性抗体。
