Bogar B, Szakacs G, Tengerdy R P, Linden J C, Pandey A
Department of Agricultural Chemical Technology, Budapest University of Technology and Economics, Hungary.
Appl Biochem Biotechnol. 2002 Jul-Dec;102-103(1-6):453-61. doi: 10.1385/abab:102-103:1-6:453.
Ten Aspergillus oryzae strains were screened in solid substrate fermentation for alpha-amylase production on spent brewing grain (SBG) and on corn fiber. SBG proved to be a better substrate for enzyme production than corn fiber. A Plackett-Burman experimental design was used to optimize the medium composition for the best strain. Solid substrate fermentation on optimized medium with A. oryzae NRRL 1808 (=ATCC 12892) strain in stationary 500-mL Erlenmeyer flask culture yielded 4519 U of alpha-amylase/g of dry matter substrate in 3 d. The whole solid substrate fermentation material (crude enzyme, in situ enzyme) may be considered a cheap biocatalytic material for animal feed rations and for bioalcohol production from starchy materials.
在固体基质发酵中，对10株米曲霉菌株进行了筛选，以评估它们利用废啤酒糟（SBG）和玉米纤维生产α-淀粉酶的能力。结果证明，SBG是比玉米纤维更适合用于酶生产的底物。采用Plackett-Burman实验设计来优化最佳菌株的培养基组成。在500 mL三角瓶静止培养条件下，用米曲霉NRRL 1808（=ATCC 12892）菌株在优化培养基上进行固体基质发酵，3天内每克干物质底物可产生4519 U的α-淀粉酶。整个固体基质发酵产物（粗酶、原位酶）可被视为一种廉价的生物催化材料，可用于动物饲料配方以及从淀粉质材料生产生物酒精。
