米曲霉和构巢曲霉高细胞密度深层培养中α-淀粉酶的产生
alpha-Amylase production in high cell density submerged cultivation of Aspergillus oryzae and A. nidulans.
作者信息
Agger T, Spohr A B, Nielsen J
机构信息
Center for Process Biotechnology, Department of Biotechnology, Technical University of Denmark, Lyngby.
出版信息
Appl Microbiol Biotechnol. 2001 Jan;55(1):81-4. doi: 10.1007/s002530000456.
The effect of biomass concentration on the formation of Aspergillus oryzae alpha-amylase during submerged cultivation with A. oryzae and recombinant A. nidulans strains has been investigated. It was found that the specific rate of alpha-amylase formation in chemostats decreased significantly with increasing biomass concentration in the range of approx. 2-12 g dry weight kg(-1). When using a recombinant A. nidulans strain in which the gene responsible for carbon catabolite repression of the A. oryzae alpha-amylase gene (creA) was deleted, no significant decrease in the specific rate of alpha-amylase formation was observed. On the basis of the experimental results, it is suggested that the low value of the specific alpha-amylase productivity observed at high biomass concentration is caused by slow mixing of the concentrated feed solution in the viscous fermentation medium.
研究了米曲霉和重组构巢曲霉菌株在深层培养过程中生物量浓度对米曲霉α-淀粉酶形成的影响。发现在恒化器中，当生物量浓度在约2-12克干重/千克范围内增加时，α-淀粉酶形成的比速率显著降低。当使用缺失负责米曲霉α-淀粉酶基因（creA）碳代谢物阻遏的基因的重组构巢曲霉菌株时，未观察到α-淀粉酶形成比速率的显著降低。根据实验结果，表明在高生物量浓度下观察到的α-淀粉酶比生产率较低是由于浓缩进料溶液在粘性发酵培养基中混合缓慢所致。
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