Dreier K H, Choi H S, Hager H
Histochemistry. 1975 Dec 8;45(3):191-201. doi: 10.1007/BF00507694.
The corpus luteum periodicum (c.l.p.) of the bitch was studied using enzyme histochemistry, clinical and biochemical methods. Assessment of variations of the serum progesterone level during different stages of the ovarian cycle and the study of enzymatic activity of the luteal tissue furnished informations on vitality and biosynthetic capacity of luteinizing cells: The period of functional activity of the c.l.p. nearly ceased about day 60 of the cycle (proestrus included) in the animals examined. In the light microscope the neo-synthesis of progesterone in the c.l.-tissue can be made visible by histochemical demonstration of the activity of Delta(5)-3 beta-hydroxysteroiddehydrogenase (3 beta-HSD) only up to a progesterone level exceeding 6.7 ng/ml plasma. The data obtained contribute to our baseline knowledge of the mechanism involved in the etiology of pseudopregnancylike conditions in the bitch.
采用酶组织化学、临床和生化方法对母犬的周期性黄体（c.l.p.）进行了研究。通过评估卵巢周期不同阶段血清孕酮水平的变化以及黄体组织的酶活性，获得了有关黄体化细胞活力和生物合成能力的信息：在所检查的动物中，周期性黄体的功能活动期在周期第60天左右（包括发情前期）几乎停止。在光学显微镜下，只有当血浆孕酮水平超过6.7 ng/ml时，通过组织化学显示Δ5-3β-羟基类固醇脱氢酶（3β-HSD）的活性，才能观察到黄体组织中孕酮的新合成。所获得的数据有助于我们了解母犬假孕样病症病因机制的基础知识。
