Fleigel Jeffery, Sedwick Jack, Kornberg Lori J
Department of Otolaryngology, University of Florida College of Medicine, Gainesville 32610, USA.
Otolaryngol Head Neck Surg. 2002 Oct;127(4):271-8. doi: 10.1067/mhn.2002.127414.
We sought to assess the effect of extracellular matrix and hepatocyte growth factor/scatter factor (HGF/SF) on the growth and motility of cultured squamous cell carcinoma of the head and neck (SCCHN) cells.
Cultured cells were incubated in the presence of HGF/SF. The effect of HFG/SF on cell growth, motility, and phosphorylation of the signaling proteins FAK and Erk was determined.
HGF/SF is both mitogenic and motogenic to the human SCCHN cell line FaDu. Incubation of FaDu cells in the presence of HGF/SF led to a rapid increase in phosphorylation of both FAK and the growth-promoting kinase Erk. HGF/SF-induced phosphorylation of FAK and Erk was observed in both detached and attached SCCHN cells. However, phosphorylation was much greater in attached cells.
The mitogenic and motogenic activities of HGF/SF may contribute to the pathogenesis of SCCHN in vivo.
我们试图评估细胞外基质和肝细胞生长因子/散射因子（HGF/SF）对培养的头颈部鳞状细胞癌（SCCHN）细胞生长和运动的影响。
将培养的细胞在HGF/SF存在的情况下进行孵育。测定HFG/SF对细胞生长、运动以及信号蛋白FAK和Erk磷酸化的影响。
HGF/SF对人SCCHN细胞系FaDu具有促有丝分裂和促运动作用。在HGF/SF存在的情况下孵育FaDu细胞导致FAK和促生长激酶Erk的磷酸化迅速增加。在分离的和贴壁的SCCHN细胞中均观察到HGF/SF诱导的FAK和Erk磷酸化。然而，贴壁细胞中的磷酸化程度要高得多。
HGF/SF的促有丝分裂和促运动活性可能在体内SCCHN的发病机制中起作用。
