Totan Alexandra Ripszky, Greabu Maria
Department of Biochemistry, University of Medicine and Pharmacy Carol Davila, Bucharest, Romania.
Acta Pol Pharm. 2002 Jul-Aug;59(4):307-11.
We have studied Na/K-ATPase and Mg-ATPase activities in red blood cells of diabetic rats treated in vivo with sodium vanadate. To our knowledge the effect of in vivo vanadate treatment on these two enzymes has not been studied. Red blood cell Na/K-ATPase plays a central role in the regulation of intra- and extra cellular cation homeostasis. Alteration of this transport enzymes is thought to be linked to several complications of diabetes mellitus: hypertension, nephropathy, peripherical neuropathy and microangiopathy. An Mg2+-dependent ATPase activity located in the erythrocyte membrane appears to be responsible for controlling the smoothing of echinocytic erythrocytes to discocytes and stomatocytes. Our results show that in alloxan diabetes activities of both ATPases are reduced (especially the activity of Na/K-ATPase). Vanadate treatment of normal animals reduced the activities of both enzymes: with 33.08% for Na/K-ATPase and 22.76% for Mg-ATPase. Vanadate treatment of diabetic animals did not affect significantly the inhibition process for Na/K-ATPase. For Mg-ATPase we have obtained a significant cumulative inhibition. These results stand out the different functions and physiologic control mechanism of these two ionic pump in red blood cells.
我们研究了用钒酸钠对糖尿病大鼠进行体内治疗后其红细胞中的钠钾-ATP酶和镁-ATP酶活性。据我们所知，尚未对体内钒酸盐治疗对这两种酶的影响进行研究。红细胞钠钾-ATP酶在调节细胞内外阳离子稳态中起核心作用。这种转运酶的改变被认为与糖尿病的几种并发症有关：高血压、肾病、周围神经病变和微血管病变。位于红细胞膜上的一种镁离子依赖性ATP酶活性似乎负责控制棘状红细胞向盘状红细胞和口形红细胞的平滑转化。我们的结果表明，在四氧嘧啶糖尿病中，两种ATP酶的活性均降低（尤其是钠钾-ATP酶的活性）。对正常动物进行钒酸盐治疗会降低两种酶的活性：钠钾-ATP酶降低33.08%，镁-ATP酶降低22.76%。对糖尿病动物进行钒酸盐治疗对钠钾-ATP酶的抑制过程没有显著影响。对于镁-ATP酶，我们获得了显著的累积抑制作用。这些结果突出了这两种离子泵在红细胞中的不同功能和生理控制机制。
