Levina L Sh, Sereda L A, Kovalenko A D, Drazhner T M
Prikl Biokhim Mikrobiol. 1975 Jan-Feb;11(1):25-9.
During yeast cultivation the activity of hexokinase and phosphofructokinase, the triggering enzymes of glycolysis, was measured and the total amount of nucleic acids was determined. The impoverished medium--postdistillation molasses residue--was used for yeast generation in the studies. A decline in the enzyme activity was observed, wheras the total amount of nucleic acids remained unaltered. The activity of hexokinase and phosphofructokinase increased significantly when the yeast cultivated on the molasses residue were stimulated by an addition of a small amount of molasses upon low aeration. The measurement of the activity of the above enzymes is a sensitive test and can be recommended for studying the technology of alcohol fermentation.
在酵母培养过程中,测定了糖酵解的触发酶己糖激酶和磷酸果糖激酶的活性,并测定了核酸的总量。在研究中,使用贫化培养基——蒸馏后糖蜜残渣——来培养酵母。观察到酶活性下降,而核酸总量保持不变。当在低通气条件下向以糖蜜残渣培养的酵母中添加少量糖蜜进行刺激时,己糖激酶和磷酸果糖激酶的活性显著增加。上述酶活性的测定是一种灵敏的测试方法,可推荐用于研究酒精发酵技术。