Lagunas R, Moreno E
Instituto de Investigaciones Biomédicas, CSIC, Madrid, Spain.
Yeast. 1992 Feb;8(2):107-15. doi: 10.1002/yea.320080205.
The enzymatic steps involved in the inhibition of glycolysis by 2-deoxygalactose in Saccharomyces cerevisiae have been investigated. Yeast, incubated with 2-deoxygalactose, accumulates up to 8 mM-2-deoxygalactose, 30 mM-2-deoxygalactose-1-phosphate and 0.25 mM-UDP-2-deoxygalactose and UDP-2-deoxyglucose. An inverse correlation between 2-deoxygalactose-1-phosphate content and rate of glycolysis has been observed. The intracellular concentration of glycolytic intermediates and related metabolites point to the hexokinase and phosphofructokinase steps as the targets for the inhibition of glycolysis by 2-deoxygalactose and rule out all other mechanisms that have been proposed to explain this inhibition.
已对酿酒酵母中2-脱氧半乳糖抑制糖酵解所涉及的酶促步骤进行了研究。用2-脱氧半乳糖培养的酵母会积累高达8 mM的2-脱氧半乳糖、30 mM的2-脱氧半乳糖-1-磷酸以及0.25 mM的UDP-2-脱氧半乳糖和UDP-2-脱氧葡萄糖。已观察到2-脱氧半乳糖-1-磷酸含量与糖酵解速率之间呈负相关。糖酵解中间产物和相关代谢物的细胞内浓度表明，己糖激酶和磷酸果糖激酶步骤是2-脱氧半乳糖抑制糖酵解的靶点，并排除了所有其他为解释这种抑制作用而提出的机制。
