豆科植物种子植酸酶使肌醇六磷酸去磷酸化的途径。

Pathway of dephosphorylation of myo-inositol hexakisphosphate by phytases of legume seeds.

作者信息

Greiner Ralf, Larsson Alminger Marie, Carlsson Nils-Gunnar, Muzquiz Mercedes, Burbano Carmen, Cuadrado Carmen, Pedrosa Mercedes M, Goyoaga Carmen

机构信息

Centre for Molecular Biology, Federal Research Centre for Nutrition, Haid-und-Neu-Strasse 9, D-76131 Karlsruhe, Germany.

出版信息

J Agric Food Chem. 2002 Nov 6;50(23):6865-70. doi: 10.1021/jf025620t.

DOI:10.1021/jf025620t

PMID:12405789

Abstract

Using a combination of high-performance ion chromatography analysis and kinetic studies, the pathway of dephosphorylation of myo-inositol hexakisphosphate by the phytases purified from faba bean and lupine seeds, respectively, was established. The data demonstrate that the legume seed phytases under investigation dephosphorylate myo-inositol hexakisphosphate in a stereospecific way. The phytase from faba bean seeds and the phytase LP2 from lupine seeds degrade phytate by sequential removal of phosphate groups via D-Ins(1,2,3,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2) to finally Ins(2)P, whereas the phytases LP11 and LP12 from lupine seeds generate the final degradation product Ins(2)P via D-Ins(1,2,4,5,6)P(5), D-Ins(1,2,5,6)P(4), D-Ins(1,2,6)P(3), and D-Ins(1,2)P(2).

摘要

通过结合高效离子色谱分析和动力学研究，分别确定了从蚕豆和羽扇豆种子中纯化的植酸酶使肌醇六磷酸去磷酸化的途径。数据表明，所研究的豆科植物种子植酸酶以立体特异性方式使肌醇六磷酸去磷酸化。蚕豆种子中的植酸酶和羽扇豆种子中的植酸酶LP2通过依次去除磷酸基团，经由D-Ins(1,2,3,5,6)P(5)、D-Ins(1,2,5,6)P(4)、D-Ins(1,2,6)P(3)和D-Ins(1,2)P(2)将植酸降解为最终产物Ins(2)P，而羽扇豆种子中的植酸酶LP11和LP12则通过D-Ins(1,2,4,5,6)P(5)、D-Ins(1,2,5,6)P(4)、D-Ins(1,2,6)P(3)和D-Ins(1,2)P(2)生成最终降解产物Ins(2)P。