Zhao Ting, Yong Xihao, Zhao Ziming, Dolce Vincenza, Li Yuan, Curcio Rosita
College of Life Science and Technology, Xinjiang University, Urumqi, China.
National Facility for Protein Science in Shanghai, Zhangjiang Lab, Shanghai Advanced Research Institute, Chinese Academy of Science, Shanghai, China.
3 Biotech. 2021 Sep;11(9):415. doi: 10.1007/s13205-021-02964-9. Epub 2021 Aug 19.
Phytic acid is abundant in seeds, roots and stems of plants, it acts as an anti-nutrient in food and feed industry, since it affects the absorption of nutrients by humans and monogastric animals. Furthermore, phosphorus produced through its decomposition by microorganisms can cause environmental pollution. Phytase degrades phytic acid generating precursors of inositol that can be used in clinical practice; in addition, phytase treatment can minimize the anti-nutritional effect of phytic acid. The use of phytase synthesized from Bacillus is more advantageous due to its high activity. Additionally, its good heat resistance under neutral conditions greatly fills the gap of commercial utilization of acid phytase. In this review, we summarize the latest research results on phytase, including its physiological and biochemical characteristics, molecular structure information, calcium effects on its catalytic activity and stability, its catalytic mechanism and molecular modification.
植酸在植物的种子、根和茎中含量丰富，在食品和饲料工业中它作为一种抗营养物质，因为它会影响人类和单胃动物对营养物质的吸收。此外，微生物分解植酸产生的磷会造成环境污染。植酸酶可降解植酸生成肌醇前体，这些前体可用于临床实践；此外，植酸酶处理可将植酸的抗营养作用降至最低。使用由芽孢杆菌合成的植酸酶更具优势，因为其活性高。此外，它在中性条件下良好的耐热性极大地填补了酸性植酸酶商业利用的空白。在这篇综述中，我们总结了关于植酸酶的最新研究成果，包括其生理生化特性、分子结构信息、钙对其催化活性和稳定性的影响、其催化机制以及分子修饰。
