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人泪液输出管中的三叶因子家族肽

TFF peptides in the human efferent tear ducts.

作者信息

Paulsen Friedrich P, Hinz Margitta, Schaudig Ulrich, Thale Andreas B, Hoffmann Werner

机构信息

Institute of Anatomy, Christian Albrechts University, Kiel, Germany.

出版信息

Invest Ophthalmol Vis Sci. 2002 Nov;43(11):3359-64.

Abstract

PURPOSE

To determine whether the lining epithelium of the human lacrimal sac and nasolacrimal duct synthesizes TFF peptides (formerly P-domain peptides, trefoil factors), a family of mucin-associated secretory peptides.

METHODS

Expression of TFF peptides in human lacrimal sac and nasolacrimal ducts was monitored by reverse transcription-polymerase chain reaction and Western blot analysis. Antisera specific for TFF peptides were used in immunohistochemical analysis to determine the presence and distribution of all three TFF peptides in epithelia of the lacrimal passage. The samples investigated originated from tissue obtained during surgery (18 patients) and postmortem tissue (10 specimens).

RESULTS

mRNA expression of TFF1 and TFF3, but not TFF2, was detected in human lacrimal sac and nasolacrimal duct. TFF1 was detected in only approximately 50% of the investigated probes, whereas TFF3 was present in all samples. Immunohistochemistry revealed TFF1 (if present) to be associated with goblet cells forming intraepithelial mucous glands. TFF3 occurred in epithelial cells of the lacrimal sac and the nasolacrimal duct as well as in the acinar cells of subepithelial serous glands, but appeared to be absent in goblet cells.

CONCLUSIONS

The epithelium of the nasolacrimal ducts synthesizes TFF3 and in some cases also TFF1. In contrast to the human conjunctiva, in which TFF3 is detectable only in goblet cells, TFF3 of the lacrimal sac and nasolacrimal duct is produced in large amounts by epithelial cells as well as by serous glands, but not-or in small amounts only-by goblet cells. This is comparable with localization of TFF3 in the major salivary glands. Thus, TFF3 may have a special function in tear transport through the lacrimal passage comparable to its function on the ocular surface, because the peptide, together with TFF1, may contribute to the rheologic properties of the tear film. Moreover, the TFF peptides may also influence epithelial healing with their motogenic properties.

摘要

目的

确定人泪囊和鼻泪管的衬里上皮是否合成三叶因子家族肽(以前称为P结构域肽、三叶因子),这是一类与粘蛋白相关的分泌肽。

方法

通过逆转录-聚合酶链反应和蛋白质印迹分析监测三叶因子家族肽在人泪囊和鼻泪管中的表达。使用针对三叶因子家族肽的抗血清进行免疫组织化学分析,以确定泪道上皮中所有三种三叶因子家族肽的存在和分布。所研究的样本来自手术中获取的组织(18例患者)和尸检组织(10个标本)。

结果

在人泪囊和鼻泪管中检测到TFF1和TFF3的mRNA表达,但未检测到TFF2的mRNA表达。仅在约50%的研究样本中检测到TFF1,而TFF3存在于所有样本中。免疫组织化学显示,TFF1(如果存在)与形成上皮内粘液腺的杯状细胞相关。TFF3存在于泪囊和鼻泪管的上皮细胞以及上皮下浆液性腺泡细胞中,但在杯状细胞中似乎不存在。

结论

鼻泪管上皮合成TFF3,在某些情况下也合成TFF1。与仅在杯状细胞中可检测到TFF3的人结膜不同,泪囊和鼻泪管的TFF3由上皮细胞以及浆液性腺大量产生,但不由杯状细胞产生或仅少量产生。这与TFF3在主要唾液腺中的定位相似。因此,TFF3在通过泪道的泪液运输中可能具有特殊功能,类似于其在眼表的功能,因为该肽与TFF1一起可能有助于泪膜的流变学特性。此外,三叶因子家族肽还可能凭借其促运动特性影响上皮愈合。

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