Langer G, Jagla W, Behrens-Baumann W, Walter S, Hoffmann W
Institut für Molekularbiologie und Medizinische Chemie, Otto-von-Guericke-Universität, Magdeburg, Germany.
Invest Ophthalmol Vis Sci. 1999 Sep;40(10):2220-4.
The objective of this study was to determine whether human conjunctival epithelium synthesizes TFF-peptides (formerly P-domain peptides, trefoil factors), a family of mucin-associated secretory peptides of the gastrointestinal tract.
Expression of TFF-peptides in human conjunctiva was monitored by reverse transcription-polymerase chain reaction analysis. Antisera specific for TFF-peptides were used for immunohistochemistry to determine the presence and distribution of TFF-peptides in human conjunctiva.
mRNA expression of TFF1 and TFF3, but not TFF2, was detected in human conjunctiva. TFF1 and TFF3 but not TFF2 are stored in conjunctival goblet cells only as revealed by immunofluorescence.
Goblet cells of the human conjunctiva synthesize TFF1 and TFF3. These peptides, together with the secretory ocular mucin MUC5AC, may contribute to the rheological properties of the tear film. They also may influence healing of corneal wounds due to their motogenic properties.
本研究的目的是确定人结膜上皮是否合成TFF肽（以前称为P结构域肽、三叶因子），这是一类胃肠道粘蛋白相关的分泌肽。
通过逆转录-聚合酶链反应分析监测TFF肽在人结膜中的表达。使用针对TFF肽的抗血清进行免疫组织化学，以确定TFF肽在人结膜中的存在和分布。
在人结膜中检测到TFF1和TFF3的mRNA表达，但未检测到TFF2的mRNA表达。免疫荧光显示，只有TFF1和TFF3而非TFF2储存于结膜杯状细胞中。
人结膜的杯状细胞合成TFF1和TFF3。这些肽与分泌性眼粘蛋白MUC5AC一起，可能有助于泪膜的流变学特性。由于它们的促运动特性，它们也可能影响角膜伤口的愈合。
