Riffel A, Brandelli A
Departamento de Ciência de Alimentos, ICTA, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil.
J Ind Microbiol Biotechnol. 2002 Nov;29(5):255-8. doi: 10.1038/sj.jim.7000307.
A Flavobacterium sp. producing a high keratinolytic activity was isolated from a poultry industry after growth on selective feather meal agar. This bacterium grew on feather meal broth, producing keratinase, and was also capable of complete degradation of raw feathers. The proteolytic activity was assessed in the presence of specific protease inhibitors. The crude enzyme showed mainly metalloprotease character. This novel isolate would have potential biotechnological use in processes involving keratin hydrolysis.
从家禽养殖场的选择性羽毛粉琼脂培养基上生长的菌群中分离出一株具有高角蛋白分解活性的黄杆菌属细菌。该细菌在羽毛粉肉汤中生长,产生角蛋白酶,并且还能够完全降解生羽毛。在特定蛋白酶抑制剂存在的情况下评估其蛋白水解活性。粗酶主要表现出金属蛋白酶的特性。这种新型分离菌株在涉及角蛋白水解的过程中具有潜在的生物技术应用价值。