Konopka A, Zakharova T, Nakatsu C
Department of Biological Sciences, Purdue University, West Lafayette, IN 47907, USA.
J Ind Microbiol Biotechnol. 2002 Nov;29(5):286-91. doi: 10.1038/sj.jim.7000322.
The kinetics of substrate degradation and bacterial growth was determined in a microbial community from a biomass recycle reactor that had been deprived of substrate feed for 0-32 days. Starvation caused changes in bacterial numbers, community composition, and physiological state. Substrate starvation for less than 1 day resulted in modest (less than threefold) changes in endogenous respiration rate, ATP content, and biomass level. During a starvation period of 32 days, there were substantial changes in microbial community composition, as assessed by denaturing gradient gel electrophoresis (DGGE) fingerprinting of PCR amplicons of a portion of the 16S rDNA or by phospholipid fatty acid (PLFA) analysis. When the starved communities were stimulated with organic nutrients, the growth kinetics was a function of the length of the starvation period. For starvation periods of 2-8 days prior to nutrient addition, there was a phase of suboptimal exponential growth (S-phase) in which the exponential growth rate was about 30% of the ultimate unrestricted growth rate. S-phase lasted for 2-8 h and then unrestricted growth occurred at rates of 0.3-0.4 h(-1). At starvation times of 12 and 20 days, a lag phase preceded S-phase and the unrestricted growth phase.
在一个生物质循环反应器的微生物群落中测定了底物降解和细菌生长的动力学，该反应器已停止底物进料0 - 32天。饥饿导致细菌数量、群落组成和生理状态发生变化。少于1天的底物饥饿导致内源性呼吸速率、ATP含量和生物量水平发生适度（小于三倍）变化。在32天的饥饿期内，通过对16S rDNA一部分的PCR扩增子进行变性梯度凝胶电泳（DGGE）指纹图谱分析或磷脂脂肪酸（PLFA）分析评估，微生物群落组成发生了显著变化。当用有机养分刺激饥饿的群落时，生长动力学是饥饿期长度的函数。在添加养分前饥饿2 - 8天的情况下，存在一个次优指数生长阶段（S期），其中指数生长速率约为最终无限制生长速率的30%。S期持续2 - 8小时，然后以0.3 - 0.4 h⁻¹的速率进行无限制生长。在饥饿12天和20天的情况下，在S期和无限制生长阶段之前有一个滞后期。
