鸡输卵管染色质的分级分离。核酸酶抗性脱氧核糖核酸。
Fractionation of chick oviduct chromatin. Nuclease-resistant deoxyribonucleic acid.
作者信息
Krall J F, Socher S H, Van N T, O'Malley B W
出版信息
Biochem J. 1975 Dec;151(3):497-503. doi: 10.1042/bj1510497.
Chromatin isolated from several chick tissues was treated with micrococcal nuclease. A limited degree of tissue specificity of chromatin DNA resistance to nuclease digestion was observed. No difference in the extent of nuclease resistance of chromatin DNA was detected during oestrogen-induced oviduct differentiation. This suggested that the amount of non-histone chromosomal protein does not play an important role in the sensitivity of chromatin DNA to nuclease digestion. Studies of nuclease resistance of chromatin DNA after dissociation and reconstitution of chromatin proteins and ethanol extraction of chromatin indicate that the histones protect the DNA from nuclease attack. Slow thermal denaturation of nuclease-resistant DNA suggests that the protected DNA sequences may be (A+T)-rich, and the (G+C)-rich satellites present in total chick DNA are sensitive to nuclease.
从几种鸡组织中分离出的染色质用微球菌核酸酶处理。观察到染色质DNA对核酸酶消化的抗性存在一定程度的组织特异性。在雌激素诱导的输卵管分化过程中，未检测到染色质DNA对核酸酶抗性程度的差异。这表明非组蛋白染色体蛋白的量在染色质DNA对核酸酶消化的敏感性中不起重要作用。对染色质蛋白解离和重组以及染色质乙醇提取后染色质DNA的核酸酶抗性研究表明，组蛋白可保护DNA免受核酸酶攻击。核酸酶抗性DNA的缓慢热变性表明，受保护的DNA序列可能富含（A+T），而鸡总DNA中存在的富含（G+C）的卫星序列对核酸酶敏感。
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