Da Ros L, Meneghetti F, Nasci C
Istituto di Biologia del Mare, Consiglio Nazionale delle Ricerche, Venezia, Italy.
Mar Environ Res. 2002 Sep-Dec;54(3-5):817-22. doi: 10.1016/s0141-1136(02)00123-x.
A field study was carried out in the Lagoon of Venice (north-east Italy) with the aim of evaluating the potential use of lysosomal destabilisation as a biomarker of anthropogenic stress in the autochthonous mussel Mytilus galloprovincialis. Two different approaches were adopted in biomonitoring six sites in the Lagoon, evaluating indigenous populations of mussels and organisms transplanted from a reference site and checked at several points in time. Lysosomal membrane stability was investigated by means of two tests: neutral red retention assay (NRRA) for evaluating haemocyte lysosomes and lysosomal latency test (LLT) for digestive cell lysosomes. Results indicate that the lysosomal response measured in haemocytes according to NRRA is a more valuable biomarker of anthropogenic stress in the framework both of passive and active biomonitoring in marine coastal environments.
在意大利东北部的威尼斯潟湖开展了一项实地研究，目的是评估溶酶体去稳定化作为本地贻贝（Mytilus galloprovincialis）人为胁迫生物标志物的潜在用途。在对潟湖中的六个地点进行生物监测时采用了两种不同的方法，评估本地贻贝种群以及从参考地点移植并在多个时间点进行检查的生物。通过两项测试研究溶酶体膜稳定性：用于评估血细胞溶酶体的中性红保留试验（NRRA）和用于消化细胞溶酶体的溶酶体潜伏试验（LLT）。结果表明，在海洋沿海环境的被动和主动生物监测框架中，根据NRRA测定的血细胞溶酶体反应是人为胁迫更有价值的生物标志物。
