Fillmann G, Watson G M, Francioni E, Readman J W, Depledge M H
Fundaçãdo Universidade Federal do Rio Grande, Depto de Oceanografia, Brazil.
Mar Environ Res. 2002 Sep-Dec;54(3-5):823-8. doi: 10.1016/s0141-1136(02)00109-5.
Urine and haemolymph can be repeatedly sampled from crabs with no (or limited) damage to the organism. Their analysis offers a measure of the animals' exposure to biologically available contaminants. Shore crabs (Carcinus maenas) were exposed to the PAHs phenanthrene and pyrene at concentrations ranging from 20 to 200 microg l(-1). After 48 h, urine and haemolymph samples were taken and analysed using ELISA and UV-fluorescence spectrophotometry. High correlations were recorded between the two sets of results from the urine analyses (r2 = 0.83 for phenanthrene and r2 = 0.88 for pyrene). Contaminant concentrations were much lower in haemolymph than in the urine. Analyses of urine taken from crabs collected from clean and contaminated sites confirm the suitability of these analyses for environmentally exposed organisms. Again, a good correlation was recorded between the ELISA and spectrofluorimetric analysis (r2 = 0.83). In this instance, difficulties were experienced with haemolymph analyses owing to a lack of sensitivity.
可以对螃蟹反复采集尿液和血淋巴样本，而对生物体没有（或只有有限的）损害。对它们的分析可以衡量动物接触生物可利用污染物的程度。将滨蟹（Carcinus maenas）暴露于浓度范围为20至200微克/升的多环芳烃菲和芘中。48小时后，采集尿液和血淋巴样本，并使用酶联免疫吸附测定（ELISA）和紫外荧光分光光度法进行分析。尿液分析的两组结果之间记录到高度相关性（菲的r2 = 0.83，芘的r2 = 0.88）。血淋巴中的污染物浓度远低于尿液中的浓度。对从清洁和受污染地点采集的螃蟹的尿液分析证实了这些分析方法适用于环境暴露生物。同样，ELISA和荧光分光光度分析之间记录到良好的相关性（r2 = 0.83）。在这种情况下，由于缺乏灵敏度，血淋巴分析遇到了困难。
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