Institute of Marine Sciences, School of Biological Sciences, University of Portsmouth, Ferry Road, Portsmouth, UK.
Mar Environ Res. 2010;69 Suppl:S46-8. doi: 10.1016/j.marenvres.2009.11.001. Epub 2009 Nov 6.
In recent years there has been increasing concern regarding the influx of pharmaceuticals into aquatic environments. One group of pharmaceuticals that has been identified as being of particular concern are the non-steroidal anti-inflammatory drugs (NSAIDs). This study examined the effects of one of the most commonly reported NSAID contaminants, diclofenac, on the physiology of Carcinus maenas. Two environmentally realistic concentrations were examined in this study (10 ng/l and 100 ng/l) at two salinities (35 and 17.5 psu). Female and male crabs were exposed to these two concentrations for 7 days. Following exposure, haemolymph samples were taken from the animals. Measurements were taken for oxyhaemocyanin and lactate concentrations. The effects of this compound on osmoregulatory capacity (OC) in C. maenas were also measured. Exposure to diclofenac had no significant effect on haemolymph lactate and oxyhaemocyanin concentrations suggesting that exposure to this compound does not elicit a stress response in this species. However, exposure to this pharmaceutical did have significant effects on both the OC and the haemolymph osmolality in C. maenas. At both salinities the data suggests that the osmoregulatory ability of C. maenas is impaired when exposed to diclofenac.
近年来，人们越来越关注药品流入水生环境的问题。已确定的一类特别值得关注的药品是 非甾体抗炎药 (NSAIDs)。本研究检测了最常报道的 NSAID 污染物之一双氯芬酸对十足目甲壳纲动物三疣梭子蟹生理机能的影响。本研究检测了两个环境现实浓度（10ng/L 和 100ng/L）在两个盐度（35 和 17.5 个盐度单位）下对双氯芬酸的影响。雌性和雄性螃蟹分别暴露于这两个浓度下 7 天。暴露后，从动物身上抽取血淋巴样本。测量氧合血蓝蛋白和乳酸盐浓度。还测量了该化合物对三疣梭子蟹渗透调节能力 (OC) 的影响。双氯芬酸暴露对血淋巴乳酸盐和氧合血蓝蛋白浓度没有显著影响，表明该化合物的暴露不会在该物种中引起应激反应。然而，这种药物的暴露对三疣梭子蟹的 OC 和血淋巴渗透压都有显著影响。在两个盐度下，数据表明，三疣梭子蟹的渗透调节能力在暴露于双氯芬酸时受到损害。
