可溶性耐药相关钙结合蛋白基因及多药耐药基因在白血病中的表达及临床意义
Expression and clinical implication of soluble resistance-associated calcium-binding protein gene and multi-drug resistance gene in leukemia.
作者信息
Li Guangyao, Tan Yaohong, Yang Chunzheng, Zhao Chunhua, Zhao Hongguo, Wang Jianxiang, Xue Yanping, Han Mingzhe, Qian Linsheng, Zhao Chunting
机构信息
National Laboratory of Experimental Hematology, Hematologic Institute, Peking Union Medical College, Chinese Academy of Medical Sciences, Tianjin 300020, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2002 Jul;24(4):370-4.
OBJECTIVE
To study the relationship between soluble resistance-related calcium-binding protein (sorcin) gene and multidrug resistance gene (mdr1), and their significance in clinical drug resistance and prognosis of acute myeloid leukemia (AML).
METHODS
Amplification of sorcin gene and mdr1 gene in K562/A02 cell detected by Northern blot, were monitored by semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) in 65 AML patients and 27 normal controls, with their relationship and clinical outcame analyzed.
RESULTS
The amplification of sorcin gene and mdr1 gene in AML patients were significantly higher than that in the normal control, which were related to clinical drug resistance and prognosis. The amplification of sorcin gene was related to the amplification of mdr1 gene in the two groups. The clinical drug resistance incidence rate and complete remission rate were 92.9% and 7.1% in sorcin(+)/mdr1(+) group. They were 8.6% and 91.4% in the sorcin(-)/mdr1(-) group (P < 0.001).
CONCLUSION
The co-amplification of sorcin and mdr1 gene can be taken as a good indicator of clinical drug resistance and prognosis of AML.
目的
研究可溶性耐药相关钙结合蛋白（索辛，sorcin）基因与多药耐药基因（mdr1）之间的关系，以及它们在急性髓系白血病（AML）临床耐药和预后中的意义。
方法
采用Northern印迹法检测K562/A02细胞中索辛基因和mdr1基因的扩增情况，应用半定量逆转录聚合酶链反应（RT-PCR）对65例AML患者和27例正常对照者进行检测，并分析它们之间的关系及临床结局。
结果
AML患者中索辛基因和mdr1基因的扩增明显高于正常对照，且与临床耐药及预后相关。两组中索辛基因的扩增与mdr1基因的扩增相关。索辛（+）/mdr1（+）组临床耐药发生率和完全缓解率分别为92.9%和7.1%。索辛（-）/mdr1（-）组分别为8.6%和91.4%（P<0.001）。
结论
索辛基因和mdr1基因的共同扩增可作为AML临床耐药和预后的良好指标。
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