烟草BY - 2细胞的环核苷酸含量
Cyclic nucleotide content of tobacco BY-2 cells.
作者信息
Richards Helen, Das Swadipa, Smith Christopher J, Pereira Louisa, Geisbrecht Alan, Devitt Nicola J, Games David E, van Geyschem Jan, Gareth Brenton A, Newton Russell P
机构信息
Biochemistry Group, Wallace Building, University of Wales Swansea, Singleton Park, Swansea SA2 8PP, UK.
出版信息
Phytochemistry. 2002 Nov;61(5):531-7. doi: 10.1016/s0031-9422(02)00266-2.
The cyclic nucleotide content of cultured tobacco bright yellow-2 (BY-2) cells was determined, after freeze-killing, perchlorate extraction and sequential chromatography, by radioimmunoassay. The identities of the putative cyclic nucleotides, adenosine 3',5'-cyclic monophosphate (cyclic AMP), guanosine 3',5'-cyclic monophosphate (cyclic GMP) and cytidine 3',5'-cyclic monophosphate (cyclic CMP) were unambiguously confirmed by tandem mass spectrometry. The potential of BY-2 cell cultures as a model system for future investigations of cyclic nucleotide function in higher plants is discussed.
通过放射免疫分析法，在对培养的烟草亮黄-2（BY-2）细胞进行冷冻杀灭、高氯酸盐提取和连续色谱分析后，测定其环核苷酸含量。通过串联质谱法明确证实了假定的环核苷酸，即3',5'-环磷酸腺苷（环磷酸腺苷）、3',5'-环磷酸鸟苷（环磷酸鸟苷）和3',5'-环磷酸胞苷（环磷酸胞苷）的身份。讨论了BY-2细胞培养作为未来研究高等植物中环核苷酸功能的模型系统的潜力。
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