Umezu Toyoshi, Ito Hiroyasu, Nagano Kimiyo, Yamakoshi Miho, Oouchi Hiroko, Sakaniwa Misao, Morita Masatoshi
Endocrine Disruptors and Dioxin Project Group, Japan.
Life Sci. 2002 Nov 22;72(1):91-102. doi: 10.1016/s0024-3205(02)02197-5.
The present study investigates the pharmacologically active constituents of rose oil, which possesses anti-conflict effects. Analysis using GC/MS revealed that rose oil contains 9 substances that were identified as myrcene, benzyl alcohol, 2-phenethyl alcohol, citronellol, geraniol, citronellyl acetate, eugenol, geranyl acetate and methyl eugenol. We examined the effects of each of these substances using the Geller and Vogel conflict tests in ICR mice. Myrcene, benzyl alcohol and citronellyl acetate did not produce any effects in either tests. Geranyl acetate and methyl eugenol produced no effect in the Geller conflict test. Geraniol and eugenol decreased the response rate during the safe period of the Geller conflict test, but did not affect the response rate during the alarm period. In contrast, 2-phenethyl alcohol and citronellol, like rose oil, produced an increasing effect on the response rate during the alarm period in the Geller conflict test. In addition, both chemicals increased the number of electric shocks mice received in the Vogel conflict test in a manner similar to that of rose oil. Given that 2-phenethyl alcohol and citronellol produced the same anti-conflict effects in both tests as rose oil, we concluded that they are the pharmacologically active constituents of anti-anxiety-like effect of rose oil.
本研究调查了具有抗冲突作用的玫瑰油的药理活性成分。气相色谱/质谱联用分析显示，玫瑰油含有9种物质，分别鉴定为月桂烯、苯甲醇、2-苯乙醇、香茅醇、香叶醇、乙酸香茅酯、丁香酚、乙酸香叶酯和甲基丁香酚。我们在ICR小鼠中使用盖勒和沃格尔冲突试验检测了这些物质各自的作用。月桂烯、苯甲醇和乙酸香茅酯在两种试验中均未产生任何作用。乙酸香叶酯和甲基丁香酚在盖勒冲突试验中未产生作用。香叶醇和丁香酚在盖勒冲突试验的安全期内降低了反应率，但在警报期内未影响反应率。相比之下，2-苯乙醇和香茅醇与玫瑰油一样，在盖勒冲突试验的警报期内对反应率产生了增加作用。此外，这两种化学物质在沃格尔冲突试验中增加小鼠接受电击的次数的方式与玫瑰油相似。鉴于2-苯乙醇和香茅醇在两种试验中产生的抗冲突作用与玫瑰油相同，我们得出结论，它们是玫瑰油抗焦虑样作用的药理活性成分。
