Niu Yingjun, Zhang Rui, Zhou Zhanyu, Wang Hongyun, Liu Fuling
Department of Ophthalmology, Affiliated Hospital, Qingdao University Medical College, Qingdao 266003, China.
Zhonghua Yan Ke Za Zhi. 2002 Sep;38(9):530-4.
To evaluate the therapeutic effect of basic fibroblast growth factor (bFGF) injected into the vitreous cavity on experimental retinal ischemia/reperfusion injury.
The Wistar rat model of experimental retinal ischemia/reperfusion injury was made by increasing the intraocular pressure. The rats were divided into normal, ischemia and treatment groups randomly. At the beginning of reperfusion, normal saline was injected into the vitreous cavity in ischemia group and 2 micro g of bFGF was injected into the treatment group. The histological and ultrastructural changes in retina of different time after reperfusion were observed. The retinal ganglion cell number was counted by using microscope. The thickness of inner layer of retina was measured by using Image Diagnosis System.
In the early period of retinal ischemia/reperfusion injury, the edematous status of retina of treatment group was lighter than that of the ischemia group. The retinal inner layer thickness and the RGC number of treatment group were greater than that of the ischemia group during all the post-reperfusion stages. At 168 hours after reperfusion, the thickness of the retinal nerve fiber layer and the RGC numbers of ischemia groups were obviously lower than that of the normal groups. But the differences of thickness of the nerve fiber layer and RGC number between the treatment and normal group were of no statistical importance. The nuclear membranes of RGCs were edematous and the mitochondrial cristae were unclear at 24th hour after reperfusion, the apoptotic body can be found, and the microtubules in nerve fibers became unclear and even disappeared in the ischemic group. While in the treatment group, the nucleus was clear, only part of the nuclear membrane was edematous, the cell organs were abundant and the structures of mitochondria and microtubules were clear.
Injection of bFGF into the vitreous cavity has the therapeutic effect on experimental retinal ischemia/reperfusion injury.
评估玻璃体腔内注射碱性成纤维细胞生长因子（bFGF）对实验性视网膜缺血/再灌注损伤的治疗效果。
通过升高眼压制作Wistar大鼠实验性视网膜缺血/再灌注损伤模型。将大鼠随机分为正常组、缺血组和治疗组。再灌注开始时，缺血组玻璃体腔内注射生理盐水，治疗组注射2μg bFGF。观察再灌注后不同时间视网膜的组织学和超微结构变化。用显微镜计数视网膜神经节细胞数量。用图像诊断系统测量视网膜内层厚度。
在视网膜缺血/再灌注损伤早期，治疗组视网膜水肿状态较缺血组轻。再灌注各阶段治疗组视网膜内层厚度和视网膜神经节细胞数量均大于缺血组。再灌注168小时时，缺血组视网膜神经纤维层厚度和视网膜神经节细胞数量明显低于正常组。但治疗组与正常组神经纤维层厚度和视网膜神经节细胞数量差异无统计学意义。再灌注24小时时，缺血组视网膜神经节细胞核膜水肿，线粒体嵴不清，可见凋亡小体，神经纤维微管不清甚至消失。而治疗组细胞核清晰，仅部分核膜水肿，细胞器丰富，线粒体和微管结构清晰。
玻璃体腔内注射bFGF对实验性视网膜缺血/再灌注损伤有治疗作用。
