Qiu Ping, Zhang Zuoming, Jiang Yong, Gou Qun, Wang Bing, Gou Lin, Chen Jingzao
Department of Clinical Aerospace Medicine, Faculty of Aviation Medicine, Guangzhou 510515, China.
Zhonghua Yan Ke Za Zhi. 2002 Aug;38(8):499-501.
To investigate the possible effect of infrasound on the ultra-structure and permeability of rat's blood-retinal barrier (BRB).
Ultra-structural changes of BRB were observed through the injection of lanthanum nitrate (La), which was used as a tracer to demonstrate the breakdown of the BRB, into blood vessels. Fifteen mature male rats divided into 5 groups were exposed to infrasound at a 8 Hz frequency, 130 dB sound pressure level in a pressure chamber especially designed for the experiment for 0, 1, 7, 14, 21 days, respectively.
Under the action of infrasound, along with the prolongation of exposure, the damage of BRB was severer and severer. On the 1st day, there was no significant change in La leakage. On the 7th day, La diffused in the interphotoreceptor space at nuclear level. On the 14th day, La granules could be seen in the space of nervous cells. Finally, on the 21st day, La was found between synapses, synapses and nerve cells, as well as between the nerve cells and supporting cells, then sometimes reached vitreous body. Under the electron microscope, there were no significant morphological changes, but changes related to metabolism, such as edematous mitochondria, dilated rough endoplasmic reticula, precipitation of glycogen grandules, widening of perinuclear space, etc.
The results thus suggest that the exposure to infrasound cause the breakdown of rat's blood-retinal barrier and visual impairment.
探讨次声对大鼠血视网膜屏障（BRB）超微结构及通透性的可能影响。
通过向血管内注射硝酸镧（La）作为示踪剂来观察BRB的超微结构变化，以证明BRB的破坏情况。将15只成年雄性大鼠分为5组，分别在专门设计的压力舱内，以8Hz频率、130dB声压级暴露于次声0、1、7、14、21天。
在次声作用下，随着暴露时间的延长，BRB的损伤越来越严重。第1天，La渗漏无明显变化。第7天，La在核水平的光感受器间隙扩散。第14天，在神经细胞间隙可见La颗粒。最后，在第21天，在突触之间、突触与神经细胞之间以及神经细胞与支持细胞之间发现了La，有时还到达玻璃体。在电子显微镜下，未见明显形态学变化，但出现了与代谢相关的变化，如线粒体水肿、粗面内质网扩张、糖原颗粒沉淀、核周间隙增宽等。
因此结果表明，暴露于次声会导致大鼠血视网膜屏障破坏和视力损害。
