[妊娠肝内胆汁淤积症中的母胎混合淋巴细胞培养]
[Maternal-fetal mixed lymphocyte culture in intrahepatic cholestasis of pregnancy].
作者信息
Dong Minyue, Xie Xing, Wang Zhengping, He Jing, Zhou Jianhong, Cheng Qi
机构信息
Women's Hospital, School of Medicine, Zhejiang University, Hangzhou 310006, China.
出版信息
Zhonghua Fu Chan Ke Za Zhi. 2002 Sep;37(9):523-5.
OBJECTIVE
To determine the maternal-fetal histocompatibility in intrahepatic cholestasis of pregnancy (ICP) by means of a morphologic method for mixed lymphocyte culture.
METHODS
Twenty-two pregnant women with ICP and 21 normal pregnant women were enrolled in the present study. Mixed lymphocyte culture was conducted using lymphocytes obtained from maternal peripheral blood and cord blood of their fetuses. The transformational rates of lymphocytes were calculated and compared between normal and ICP-complicated pregnancies.
RESULTS
The transformational rate in ICP was (24 +/- 5)%, while that in normal pregnant women was (36 +/- 9)%, the difference was significant between two groups (P < 0.001). The presence of pregnancy-induced hypertension did not affect the transformational rates in ICP group (P > 0.05). The transformational rate of lymphocyte in ICP did not correlate with cholylglycine level in serum significantly (P > 0.05).
CONCLUSION
Decreased maternal-fetal lymphocyte reaction is demonstrated in ICP and it may play an etiological role.
目的
通过混合淋巴细胞培养的形态学方法确定妊娠期肝内胆汁淤积症（ICP）患者的母胎组织相容性。
方法
本研究纳入22例ICP孕妇和21例正常孕妇。采用孕妇外周血淋巴细胞和胎儿脐血淋巴细胞进行混合淋巴细胞培养。计算淋巴细胞转化率，并比较正常妊娠和ICP合并妊娠之间的差异。
结果
ICP组淋巴细胞转化率为（24±5）%，正常孕妇组为（36±9）%，两组差异有统计学意义（P<0.001）。妊娠期高血压的存在不影响ICP组的淋巴细胞转化率（P>0.05）。ICP患者淋巴细胞转化率与血清甘氨胆酸水平无明显相关性（P>0.05）。
结论
ICP患者母胎淋巴细胞反应降低，可能在病因学上起作用。
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