Petersen Nickolaj J, Mogensen Klaus B, Kutter Jörg P
MIC-Mikroelektronik Centret, Technical University of Denmark, DK-8000 Lyngby, Denmark.
Electrophoresis. 2002 Oct;23(20):3528-36. doi: 10.1002/1522-2683(200210)23:20<3528::AID-ELPS3528>3.0.CO;2-5.
A microfluidic device with integrated waveguides and a long path length detection cell for UV/Vis absorbance detection is presented. The 750 microm U-cell detection geometry was evaluated in terms of its optical performance as well as its influence on efficiency for electrophoretic separations in the microdevice. Stray light was found to have a strong effect on both, the sensitivity of the detection and the available linear range. The long path length U-cell showed a 9 times higher sensitivity when compared to a conventional capillary electrophoresis (CE) system with a 75 microm inner diameter (ID) capillary, and a 22 times higher sensitivity than with a 50 microm ID capillary. The linear range was comparable to that achieved in a 75 microm ID capillary and more than twice as large as in a 50 microm ID capillary. The use of the 750 microm U-cell did not contribute significantly to band broadening; however, a clear quantification was made difficult by the convolution of several other band broadening sources.
本文介绍了一种集成波导和长光程检测池的微流控装置，用于紫外/可见吸光度检测。对750微米的U型检测池结构进行了光学性能评估，以及其对微流控装置中电泳分离效率的影响。发现杂散光对检测灵敏度和可用线性范围均有强烈影响。与内径75微米的传统毛细管电泳（CE）系统相比，长光程U型检测池的灵敏度高9倍，比内径50微米的毛细管高22倍。线性范围与内径75微米的毛细管相当，是内径50微米毛细管的两倍多。使用750微米的U型检测池对谱带展宽没有显著影响；然而，由于其他几种谱带展宽源的卷积，使得清晰的定量分析变得困难。
