新生大鼠培养心肌细胞簇中自发活动的离子决定因素。

Ionic determinants of spontaneous activity in clusters of cultured cardiac cells from newborn rats.

作者信息

Schanne O F, Rivard C, Doyon G

出版信息

Can J Physiol Pharmacol. 1975 Dec;53(6):1209-13. doi: 10.1139/y75-170.

Abstract

The spontaneous activity of cell clusters derived from ventricle cells of newborn rats was studied using a recording television microscope. The influence of varying concentrations of sodium, potassium, calcium, tetrodotoxin (TTX), and that of 2 mM MnCl2 was tested. The spontaneous activity of the cell clusters persisted in TTX but it was abolished by Mn. The beating rate increased when [Ca]0 and [Na]0 were changed from 0.3 mM to 3.0 mM and from 30 mM to 75 mM; it decreased with a change of [Na]0 from 75 mM to 142 mM. It is concluded that electrogenesis in their behavior to very young embryonic rat heart cells or cells of the rabbit sinoauricular node.

摘要

使用记录电视显微镜研究了新生大鼠心室细胞来源的细胞簇的自发活动。测试了不同浓度的钠、钾、钙、河豚毒素（TTX）以及2 mM MnCl₂ 的影响。细胞簇的自发活动在TTX中持续存在，但被Mn消除。当[Ca]₀ 和[Na]₀ 从0.3 mM变为3.0 mM以及从30 mM变为75 mM时，搏动率增加；随着[Na]₀ 从75 mM变为142 mM，搏动率降低。得出结论，它们的行为中的电发生与非常年轻的胚胎大鼠心脏细胞或兔窦房结细胞的电发生情况相同。