Furtado Agnelo, Henry Robert
Cooperative Research Centre for Molecular Plant Breeding, Centre for Plant Conservation, Genetics, Southern Cross University, Military Road, Lismore, NSW 2480, Australia.
Anal Biochem. 2002 Nov 1;310(1):84-92. doi: 10.1016/s0003-2697(02)00281-6.
The gene encoding the green fluorescent protein (GFP) has been widely used in studies of gene expression. The GFP can be detected nondestructively in living cells or tissues by the green fluorescence of the protein under blue light. Solutions of enhanced GFP (EGFP) of known concentration were filled in glass capillaries and used to calibrate a method for quantitative determination of EGFP or GFP-S65T in plant cells. Images captured by a digital camera were analyzed to determine the linear range for measurement of EGFP expression. The value of the method was illustrated by analysis of the relative levels of GFP expression under control of different promoters in aleurone cells of barley.
编码绿色荧光蛋白（GFP）的基因已广泛应用于基因表达研究。在蓝光照射下，可通过该蛋白的绿色荧光在活细胞或组织中无损检测GFP。将已知浓度的增强型GFP（EGFP）溶液填充到玻璃毛细管中，并用于校准一种定量测定植物细胞中EGFP或GFP-S65T的方法。分析数码相机拍摄的图像，以确定测量EGFP表达的线性范围。通过分析大麦糊粉层细胞中不同启动子控制下GFP表达的相对水平，说明了该方法的价值。
