Pirpignani María L, Rivera Elena, Hellman Ulf, Biscoglio de Jiménez Bonino Mirtha
Instituto de Química y Fisicoquímica Biológicas (UBA-CONICET), Junin 956, Universidad de Buenos Aires, Buenos Aires, Argentina.
Arch Biochem Biophys. 2002 Nov 15;407(2):224-30. doi: 10.1016/s0003-9861(02)00554-4.
Vespid venoms contain Antigen 5, an important allergen whose primary structure and immunological behavior have been extensively studied from venoms of vespids of the Northern Hemisphere. We report herein structural and immunological aspects of Antigen 5 from Polybia scutellaris subspecies rioplatensis (vulgar name: camoati) found in South America. Mast cell degranulation, histamine release, and IgE induction experiments performed in mice allow us to suggest that P. scutellaris Antigen 5 is a variant with reduced IgE response and anaphylactic activity. Sequence data indicate that the protein has a 72.5-90.3% similarity to that of members of the vespid Antigen 5 family with an already known primary structure. Moreover, results suggest that the protein-a new member of an extracellular protein superfamily-could be a good candidate for immunotherapy related to vespid allergy.
胡蜂毒液中含有抗原5，这是一种重要的过敏原，其一级结构和免疫行为已在北半球胡蜂毒液中得到广泛研究。我们在此报告了从南美洲发现的黄胸木胡蜂（俗名：卡莫阿蒂）中提取的抗原5的结构和免疫方面的情况。在小鼠身上进行的肥大细胞脱颗粒、组胺释放和IgE诱导实验使我们认为，黄胸木胡蜂抗原5是一种IgE反应和过敏活性降低的变体。序列数据表明，该蛋白与已知一级结构的胡蜂抗原5家族成员具有72.5-90.3%的相似性。此外，结果表明，该蛋白作为细胞外蛋白超家族的新成员，可能是胡蜂过敏免疫治疗的良好候选物。
