DelVecchio Vito G, Kapatral Vinayak, Elzer Philip, Patra Guy, Mujer Cesar V
Institute of Molecular Biology and Medicine, University of Scranton, Scranton, PA 18510, USA.
Vet Microbiol. 2002 Dec 20;90(1-4):587-92. doi: 10.1016/s0378-1135(02)00238-9.
The genome of Brucella melitensis strain 16M was sequenced and contained 3,294,931 bp distributed over two circular chromosomes. Chromosome I was composed of 2,117,144 bp and chromosome II has 1,177,787 bp. A total of 3,198 ORFs were predicted. The origins of replication of the chromosomes are similar to each other and to those of other alpha-proteobacteria. Housekeeping genes such as those that encode for DNA replication, protein synthesis, core metabolism, and cell-wall biosynthesis were found on both chromosomes. Genes encoding adhesins, invasins, and hemolysins were also identified.
对布鲁氏菌16M菌株的基因组进行了测序，其基因组包含3294931个碱基对，分布在两条环状染色体上。染色体I由2117144个碱基对组成，染色体II有1177787个碱基对。共预测出3198个开放阅读框。两条染色体的复制起点彼此相似，且与其他α-变形菌的复制起点相似。在两条染色体上均发现了一些管家基因，如编码DNA复制、蛋白质合成、核心代谢和细胞壁生物合成的基因。还鉴定出了编码黏附素、侵袭素和溶血素的基因。
