Kopnin B P, Stavrovskaia A A
Genetika. 1975;11(2):118-24.
The comparative study of the frequency of colcemid-induced aneuploidy and polyploidy in cultured normal and transformed cells of Djungarian hamster is described. The occurrence of variants with changed chromosome number is much higher in populations of SV40-transformed cell line (4/21) than in normal embryonic cultures. In transformed lines of Djungarian and Chinese hamsters (4/21 and V-79) the frequency of cells with changed chromosome number was found to be depend on the culture density: the percentage of polyploids was 4-5-fold higher when the number of seeded cells was 2-fold lower. The highest number (18-29%) of hypermodal cells was produced at drug concentrations of 0.02--0.025 mkg/ml. The percentage of polyploids under these conditions reached 10--20. At further increaseof colcemid concentrations the proportion of polyploid cells increased. In Djungarian hamster embryonic cell cultures there were single cells with changed chromosome numbers at a concentrarion of the drug of 0.015-0.1 mkg/ml.
本文描述了在体外培养的正常和经转化的黑线仓鼠细胞中，秋水仙酰胺诱导非整倍体和多倍体频率的比较研究。在SV40转化细胞系群体中（4/21），染色体数目改变的变异体出现频率比正常胚胎培养物中高得多。在黑线仓鼠和中国仓鼠的转化细胞系（4/21和V - 79）中，发现染色体数目改变的细胞频率取决于培养密度：接种细胞数降低2倍时，多倍体细胞的百分比高出4 - 5倍。药物浓度为0.02 - 0.025微克/毫升时，超数细胞产生的数量最多（18 - 29%）。在这些条件下，多倍体细胞的百分比达到10 - 20。随着秋水仙酰胺浓度进一步增加，多倍体细胞的比例也增加。在黑线仓鼠胚胎细胞培养物中，药物浓度为0.015 - 0.1微克/毫升时出现了单个染色体数目改变的细胞。
