Mock S, Shen X, Tamvakopoulos C
Merck Research Laboratories, Rahway, NJ 07065, USA.
Rapid Commun Mass Spectrom. 2002;16(22):2142-7. doi: 10.1002/rcm.847.
A method is described for the determination of melanotan-II (MT-II), a synthetic cyclic heptapeptide analog of alpha-melanocyte-stimulating hormone (alpha-MSH), in rat plasma by liquid chromatography/tandem mass spectrometry (LC/MS/MS). The analyte is recovered from plasma by solid-phase extraction (SPE) and subsequently analyzed by LC/MS/MS. A SPE procedure using OASIS 96-well plates is used for extraction of MT-II from rat plasma. Using the described approach a limit of quantitation of 5 ng/mL was achieved in rat plasma. This level of sensitivity allowed the determination of pharmacokinetic parameters, following intravenous administration of MT-II in rat.
本文描述了一种通过液相色谱/串联质谱法（LC/MS/MS）测定大鼠血浆中黑素皮质素-II（MT-II）的方法，MT-II是α-黑素细胞刺激素（α-MSH）的一种合成环七肽类似物。通过固相萃取（SPE）从血浆中回收分析物，随后用LC/MS/MS进行分析。使用OASIS 96孔板的SPE程序从大鼠血浆中提取MT-II。采用所述方法，大鼠血浆中的定量限达到5 ng/mL。这种灵敏度水平使得在大鼠静脉注射MT-II后能够测定药代动力学参数。
