Maggiora Marina, Dianzani Mario Umberto, Rossi Maria Armida
Department of Experimental Medicine and Oncology, Section of General Pathology, University of Turin, Corso Raffaello 30, 10125 Turin, Italy.
Cell Biochem Funct. 2002 Dec;20(4):303-7. doi: 10.1002/cbf.975.
Our work analysed the effect of 4-hydroxynonenal (HNE), a chemotactic aldehydic end-product of lipid peroxidation, on exocytosis in HL-60 cells. We measured the release of beta-glucuronidase, an enzyme of azurophil granules, from the cells incubated at 37 degrees C for 10 min in the presence of HNE concentrations ranging between 10(-8) and 10(-5) M. The release of lactate dehydrogenase was assayed to test cell viability. HNE (1 microM) was able to induce a significant and strong stimulation of beta-glucuronidase secretion without leading to cytotoxic effects. The finding that HNE could increase the exocytotic secretion from HL-60 cells together with its known chemotactic property supports the hypothesis that this lipid peroxidation product may play an important role as a chemical mediator of inflammation; moreover it is noteworthy that micromolar concentrations of HNE have actually been found in exudates from acute and chronic inflammations.
我们的研究分析了4-羟基壬烯醛（HNE），一种脂质过氧化的趋化性醛类终产物，对HL-60细胞胞吐作用的影响。我们测定了在37℃下于不同浓度HNE（10^(-8)至10^(-5) M）存在下孵育10分钟的细胞中嗜天青颗粒酶β-葡萄糖醛酸酶的释放。检测乳酸脱氢酶的释放以测试细胞活力。1微摩尔的HNE能够显著且强烈地刺激β-葡萄糖醛酸酶的分泌，而不会导致细胞毒性作用。HNE能够增加HL-60细胞的胞吐分泌这一发现，连同其已知的趋化特性，支持了这样一种假设，即这种脂质过氧化产物可能作为炎症的化学介质发挥重要作用；此外，值得注意的是，在急性和慢性炎症的渗出物中实际上已发现微摩尔浓度的HNE。
