Thirunavukkarasu C, Prince Vijeya Singh J, Thangavel M, Selvendiran K, Sakthisekaran D
Department of Medical Biochemistry, Dr ALM PG Institute of Basic Medical Sciences, University of Madras, Chennai-600 113, India.
Cell Biochem Funct. 2002 Dec;20(4):347-56. doi: 10.1002/cbf.979.
The dietary administration of selenium (sodium selenite; 4 p.p.m.) daily has been found to be highly effective in reducing the incidence of cancer induced by N-nitrosodiethylamine (DEN) in Wistar strain rats. Selenium treatment either before initiation, during initiation and selection/phenobarbital promotion phases of hepatocarcinogenesis has been found to be effective in elevating hepatic microsomal cytochrome b(5), NADPH-cytochrome C reductase and cytosolic aryl hydrocarbon hydroxylase activities to a statistically significant level measured either in the hyperplastic nodule or in the surrounding liver tissues compared to control animals. Moreover, selenium treatment throughout the study, decreases the cytosolic glutathione S-transferase and microsomal UDP-glucuronyl transferase activities by a significant degree when compared to control rats. Alterations in glutathione metabolizing enzyme activities (glutathione reductase, gamma-glutamyl transpeptidase, gamma-glutamylcysteine synthetase and glucose-6-phosphate dehydrogenase) were also observed in selenium-treated groups. Our results confirm the fact that selenium is particularly protective in limiting the action of DEN during the initiation phase of hepatocarcinogenesis.
每日膳食给予硒（亚硒酸钠；4 ppm）已被发现对降低Wistar品系大鼠中由N-亚硝基二乙胺（DEN）诱导的癌症发病率非常有效。在肝癌发生的起始前、起始期间以及选择/苯巴比妥促进阶段进行硒处理，已发现与对照动物相比，在增生性结节或周围肝组织中测量时，可有效将肝微粒体细胞色素b(5)、NADPH-细胞色素C还原酶和胞质芳烃羟化酶活性提高到具有统计学意义的水平。此外，与对照大鼠相比，在整个研究过程中进行硒处理可显著降低胞质谷胱甘肽S-转移酶和微粒体UDP-葡萄糖醛酸转移酶活性。在硒处理组中还观察到谷胱甘肽代谢酶活性（谷胱甘肽还原酶、γ-谷氨酰转肽酶、γ-谷氨酰半胱氨酸合成酶和葡萄糖-6-磷酸脱氢酶）的改变。我们的结果证实了硒在肝癌发生起始阶段对限制DEN的作用具有特别保护作用这一事实。
