Dvorzhak M, Travnik P
Arkh Anat Gistol Embriol. 1975 Jun;69(7):26-9.
The presence of exogenous peroxidase of the rat's eggs during cleavage at the 1-, 2-, 8-cellular stages and the blastocysts were studied after 30 minute incubation with horseradish peroxidase (molecular weight 40.000, approximate size of the molecule 40 A). The activity of exogenous peroxidase in the area of zona pellucida was established in the perivitelline space, in the cytoplasm - in the pinocytosis vesicles, in certain greater vacuoles and in certain secondary lysosomes. At the stage of blastocyst the penetration of exogenous protein was greater than at earlier stages. The reaction product was found here in the intercellular space and in the blastocyst cavity. The obtained data suggest possible penetration of the protein from the surrounding area into the egg during its passing the uterine tube. The substances influencing the differentiation of the egg or teratogens may penetrate the egg in the same way.
在将大鼠卵与辣根过氧化物酶（分子量40000，分子大小约40埃）孵育30分钟后，研究了1细胞期、2细胞期、8细胞期卵裂期及囊胚期大鼠卵中外源过氧化物酶的存在情况。已确定在透明带区域、卵周隙、细胞质中的胞饮小泡、某些较大的液泡和某些次级溶酶体中存在外源过氧化物酶活性。在囊胚期，外源蛋白质的穿透比早期阶段更大。在此处的细胞间隙和囊胚腔中发现了反应产物。所获得的数据表明，蛋白质在卵通过输卵管时可能从周围区域穿透到卵中。影响卵分化的物质或致畸剂可能以相同的方式穿透卵。
