Kanno Toru, Yamakawa Makoto, Yoshida Kazuo, Sakamoto Kenichi
Department of Exotic Diseases, National Institute of Animal Health, Kodaira, Tokyo, Japan.
Virus Genes. 2002 Oct;25(2):119-25. doi: 10.1023/a:1020103700108.
The complete nucleotide sequence of the foot-and-mouth disease virus (FMDV) O/JPN/2000 strain, the PanAsia strain, was determined by cycle sequencing and primer walking. The 5' end of the genome upstream from homopolymeric poly(C) tract (S-fragment) was 367 nucleotides in length, and the remainder of the genome (L-fragment), excepting the poly(A) tail, was 7808 nucleotides. The L-fragment contains a single open reading frame of 6996 nucleotides terminating at a UAA codon 96 bases from the 3' poly(A) sequence. Comparison of sequences shows that the length of the structural and non-structural protein coding regions are identical to those in the O1/Kaufbeuren strain, and no striking differences such as deletion or insertion were observed between them.
通过循环测序和引物步移法测定了口蹄疫病毒（FMDV）泛亚毒株O/JPN/2000株的完整核苷酸序列。基因组5'端同聚多聚（C）序列（S片段）上游长度为367个核苷酸，基因组其余部分（L片段），除了多聚（A）尾，长度为7808个核苷酸。L片段包含一个6996个核苷酸的单一开放阅读框，终止于距3'多聚（A）序列96个碱基处的UAA密码子。序列比较表明，结构蛋白和非结构蛋白编码区的长度与O1/Kaufbeuren株相同，且未观察到它们之间有明显差异，如缺失或插入。
