[人肝外生长型肝癌细胞系EGHC - 9901的建立与鉴定]
[Establishment and characterization of human extrahepatic growing hepatocellular carcinoma cell line EGHC-9901].
作者信息
Wu Xiaopeng, Wang Zhanmin, Liu Bo, Liu Jun, Gao Yingmao, Li Zhaoting, Liu Chunsheng
机构信息
Department of General Surgery, Qilu Hospital, Shandong University, Ji'nan 250012, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2002 Aug;40(8):616-7.
OBJECTIVE
To establish a new extrahepatic growing hepatocellular carcinoma cell line.
METHODS
A specimen from extrahepatic growing hepatocellular carcinoma was cultured in vitro. Cancer cells were studied morphologically and subjected to karyotype analysis, DNA analysis, and tumor formation evaluation.
RESULTS
Morphological observation and functional analysis showed that their features were similar to those of HCC. Chromosomes with a variation of 76 approximately 104 were able to secret AFP in vitro and to form bile canaliculi with microvilli.
CONCLUSION
EGHC-9901 cell line has characteristics of the extrahepatic growing hepatocellular carcinoma.
目的
建立一种新的肝外生长型肝癌细胞系。
方法
将肝外生长型肝癌标本进行体外培养。对癌细胞进行形态学研究，并进行核型分析、DNA分析及肿瘤形成评估。
结果
形态学观察和功能分析表明，其特征与肝癌相似。染色体数目在76至104之间变化，能够在体外分泌甲胎蛋白，并形成带有微绒毛的胆小管。
结论
EGHC - 9901细胞系具有肝外生长型肝癌的特征。
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