Lewis Edwin E, Shapiro-Ilan David I
Department of Entomology, Price Hall, Virginia Tech, Blacksburg, VA 24061-0319, USA.
J Invertebr Pathol. 2002 Sep;81(1):25-32. doi: 10.1016/s0022-2011(02)00115-5.
The entomopathogenic nematodes Heterorhabditis bacteriophora, Steinernema carpocapsae, Steinernema glaseri, and Steinernema feltiae were exposed to freezing while inside their hosts. Survival was assessed by observing live and dead nematodes inside cadavers and by counting the infective juveniles (IJs) that emerged after freezing. We (1) measured the effects of 24h of freezing at different times throughout the course of an infection, (2) determined the duration of freezing entomopathogenic nematodes could survive, (3) determined species differences in freezing survival. Highest stage-specific survival was IJs for S. carpocapsae, and adults for H. bacteriophora. When cadavers were frozen two or three days after infection, few IJs emerged from them. Freezing between five and seven days after infection had no negative effect on IJ production. No decrease in IJ production was measured for H. bacteriophora after freezing. H. bacteriophora also showed improved survival inside versus outside their host when exposed to freezing.
将昆虫病原线虫嗜菌异小杆线虫、小卷蛾斯氏线虫、格氏斯氏线虫和长尾斯氏线虫置于宿主体内时进行冷冻处理。通过观察虫尸内活线虫和死线虫以及统计冷冻后出现的感染性幼虫（IJ）来评估存活率。我们（1）测量了在感染过程中不同时间进行24小时冷冻的影响，（2）确定了昆虫病原线虫能够存活的冷冻持续时间，（3）确定了冷冻存活率的物种差异。特定阶段的最高存活率，对于小卷蛾斯氏线虫是感染性幼虫，对于嗜菌异小杆线虫是成虫。当在感染后两天或三天冷冻虫尸时，从中出来的感染性幼虫很少。在感染后五至七天进行冷冻对感染性幼虫的产生没有负面影响。对于嗜菌异小杆线虫，冷冻后未检测到感染性幼虫产生量的下降。当暴露于冷冻环境时，嗜菌异小杆线虫在宿主体内的存活率也高于体外。
