Peltoniemi J, Setälä N, Broberg E, Röyttä M, Hukkanen V, Salmi A A, Erälinna J-P
Department of Virology, University of Turku, Kiinamyllynkatu 13, 20520, Turku, Finland.
J Neuroimmunol. 2002 Nov;132(1-2):83-92. doi: 10.1016/s0165-5728(02)00312-0.
Linomide (quinoline-3-carboxamide) is an immunomodulator with diverse effects on the immune system. Its beneficial effects on experimental autoimmune disease models have been linked to downregulation of Th1 cytokines and altered macrophage functions. We studied this effect of downregulation of Th1-type of immune response on Semliki Forest A7 virus infection in experimental autoimmune encephalomyelitis (EAE) susceptible Th1-prone SJL mice and in EAE-resistant Th2-prone BALB/c mice. We aimed at addressing the target-cell population of Linomide responsible for this Th1 downregulation. Treatment with Linomide led to increased virus infection in brain and this effect coincided with decreased production of IL-12 and IFN-gamma from stimulated spleen cells in SJL mice. In contrast, IL-12 and IFN-gamma expression were increased in Linomide-treated BALB/c mice. Treatment of infected SJL mice resulted in decreased percentage of CD11b+ and CD11c+ cells. Thus, the target cell population of Linomide may be antigen-presenting cells (APC) which are considered as candidates for regulatory cells of Th1/Th2 balance.
来那度胺（喹啉 - 3 - 甲酰胺）是一种对免疫系统有多种作用的免疫调节剂。其对实验性自身免疫病模型的有益作用与Th1细胞因子的下调及巨噬细胞功能改变有关。我们研究了在实验性自身免疫性脑脊髓炎（EAE）易感的Th1倾向型SJL小鼠和EAE抗性的Th2倾向型BALB/c小鼠中，Th1型免疫反应下调对Semliki森林A7病毒感染的影响。我们旨在确定来那度胺导致这种Th1下调的靶细胞群体。来那度胺治疗导致脑部病毒感染增加，且这一效应与SJL小鼠中受刺激的脾细胞产生的IL - 12和IFN - γ减少相吻合。相反，在接受来那度胺治疗的BALB/c小鼠中，IL - 12和IFN - γ表达增加。对感染的SJL小鼠进行治疗导致CD11b +和CD11c +细胞百分比降低。因此，来那度胺的靶细胞群体可能是抗原呈递细胞（APC），其被认为是Th1/Th2平衡调节细胞的候选者。
