Lei Hai-Yan, Zhou Bo, Zhang Yu, Hong Guo-Fan
National Center for Gene Research, Shanghai Institutes for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200233, China.
Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 2002 Nov;34(6):685-9.
Sequencing analysis of the 323 kb contig of rice chromosome 4 identified a gene cluster encoding 7 dihydroflavonol-4-reductase (DFR)-like proteins within a 56 kb region. The 7 DFR-like genes were found to be arranged in a tandem array, and all of them comprised 6 exons and 5 introns. Analysis of the predicted amino acid sequences demonstrated that these 7 proteins shared strong similarities with DFR and other enzymes of the phenylpropanoid biosynthesis pathway. RT-PCR revealed the expression pattern of the 7 genes was different in various rice tissues. The structural and functional features of these 7 DFR-like genes and their evolutionary implications are discussed.
对水稻第4号染色体323 kb重叠群的测序分析，在一个56 kb区域内鉴定出一个编码7个二氢黄酮醇-4-还原酶（DFR）样蛋白的基因簇。发现这7个DFR样基因呈串联排列，它们均由6个外显子和5个内含子组成。对预测的氨基酸序列分析表明，这7种蛋白与DFR及苯丙烷生物合成途径的其他酶具有很强的相似性。逆转录聚合酶链反应（RT-PCR）显示这7个基因在水稻不同组织中的表达模式不同。本文讨论了这7个DFR样基因的结构和功能特征及其进化意义。
