Housaindokht Mohammad R, Bahrololoom Mahmood, Tarighatpoor Shirin, Mossavi-Movahedi Ali A
Department of Chemistry, Faculty of Science, Ferdowsi University of Mashhad, Iran.
Acta Biochim Pol. 2002;49(3):703-7.
A new approach has been developed to study binding of a ligand to a macromolecule based on the diffusion process. In terms of the Fick's first law, the concentration of free ligand in the presence of a protein can be determined by the measurement of those ligands which are diffused out. This method is applied to the study of binding of methyl-orange to lysozyme in phosphate buffer of pH 6.2, at 30 degrees C. The binding isotherm was determined initially, followed by application of the Hill equation to the data obtained, then binding constant and binding capacity were estimated.
一种基于扩散过程研究配体与大分子结合的新方法已经被开发出来。根据菲克第一定律，在蛋白质存在的情况下，游离配体的浓度可以通过测量扩散出去的那些配体来确定。该方法应用于在30℃、pH 6.2的磷酸盐缓冲液中甲基橙与溶菌酶结合的研究。首先测定结合等温线，然后将希尔方程应用于所获得的数据，进而估算结合常数和结合容量。
