Xu Dan, Meng Fan-Yi, Zhang Yan
Department of Hematology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Apr;22(4):357-9.
To investigate the role of vascular endothelial growth factor (VEGF) in the pathogenesis and progression of acute myeloid leukemia (AML).
Reverse transcription-polymerase chain reaction (RT-PCR) was employed for VEGF mRNA detection. VEGF levels in the bone marrow cell cultural supernatant and plasma from normal subjects and AML patients were determined by enzyme-linked immunosorbent assay (ELISA).
VEGF mRNA expression was present in most of the AML cell lines HL-60, K562 and Raji, but not in Jurkat cells. The expression rate of VEGF mRNA in the bone marrow cells from AML patients (82%) was higher than the rates in normal subjects (30.76%) and AML patients with autologous bone marrow transplantation (41.67%). Refractory and non-refractory AML patients had higher mean plasma VEGF levels than the normal donors and AML patients with transplantation did, without significant differences observed in the latter 2 groups. With also significant difference between them, refractory and non-refractory AML groups had respectively 11.0 and 7.3-fold higher VEGF levels in the culture supernatant than the normal donor group did.
The abnormality of VEGF expression may play an important role in the development of AML, and VEGF expression might be used as one of the indexes for prognostic assessment for AML patients.
探讨血管内皮生长因子（VEGF）在急性髓系白血病（AML）发病机制及病情进展中的作用。
采用逆转录聚合酶链反应（RT-PCR）检测VEGF mRNA。通过酶联免疫吸附测定（ELISA）法测定正常人和AML患者骨髓细胞培养上清液及血浆中的VEGF水平。
大多数AML细胞系HL-60、K562和Raji中存在VEGF mRNA表达，但Jurkat细胞中无此表达。AML患者骨髓细胞中VEGF mRNA的表达率（82%）高于正常受试者（30.76%）和接受自体骨髓移植的AML患者（41.67%）。难治性和非难治性AML患者的血浆VEGF平均水平高于正常供者和接受移植的AML患者，后两组间无显著差异。难治性和非难治性AML组培养上清液中的VEGF水平分别比正常供者组高11.0倍和7.3倍，两组间也有显著差异。
VEGF表达异常可能在AML的发生发展中起重要作用，VEGF表达可作为AML患者预后评估的指标之一。
