产碱普罗威登斯菌O16的含N - 乙酰胞壁酸的O - 特异性多糖的结构

Structure of the O-specific polysaccharide of Providencia alcalifaciens O16 containing N-acetylmuramic acid.

作者信息

Kocharova Nina A, Zatonsky George V, Bystrova Olga V, Ziolkowski Andrzej, Wykrota Marianna, Shashkov Aleksander S, Knirel Yuriy A, Rozalski Antoni

机构信息

ND Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, GSP-1, Moscow, Russia.

出版信息

Carbohydr Res. 2002 Oct 8;337(18):1667-71. doi: 10.1016/s0008-6215(02)00022-8.

DOI:10.1016/s0008-6215(02)00022-8

PMID:12423969

Abstract

The O-specific polysaccharide of Providencia alcalifaciens O16 was obtained by mild-acid degradation of the lipopolysaccharide and studied by chemical methods and NMR spectroscopy, including 2D 1H,(1)H COSY, TOCSY, NOESY, and 1H,(13)C HSQC experiments. It was found that the polysaccharide contains N-acetylmuramic acid, which was isolated by solvolysis with trifluoromethanesulfonic acid and identified by the specific optical rotation and NMR spectroscopy. The following structure of the trisaccharide repeating-unit of the polysaccharide was established:

摘要

通过对嗜碱普罗威登斯菌O16脂多糖进行温和酸降解获得其O-特异性多糖，并采用化学方法和核磁共振波谱法（包括二维1H,(1)H COSY、TOCSY、NOESY和1H,(13)C HSQC实验）进行研究。结果发现该多糖含有N-乙酰胞壁酸，通过用三氟甲磺酸进行溶剂解将其分离，并通过比旋度和核磁共振波谱法进行鉴定。确定了该多糖三糖重复单元的以下结构：