产碱普罗威登斯菌O32脂多糖中含N-乙酰异Muramic酸的O-多糖结构及血清学交叉反应性

Structure of the O-polysaccharide and serological cross-reactivity of the lipopolysaccharide of Providencia alcalifaciens O32 containing N-acetylisomuramic acid.

作者信息

Bushmarinov Ivan S, Ovchinnikova Olga G, Kocharova Nina A, Toukach Filip V, Torzewska Agnieszka, Shashkov Alexander S, Knirel Yuriy A, Rozalski Antoni

机构信息

N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.

出版信息

Carbohydr Res. 2007 Feb 5;342(2):268-73. doi: 10.1016/j.carres.2006.11.027. Epub 2006 Dec 3.

DOI:10.1016/j.carres.2006.11.027

PMID:17182016

Abstract

UNLABELLED

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O32 and studied by sugar and methylation analyses, solvolysis with triflic acid, 1H and 13C NMR spectroscopy, including two-dimensional 1H,1H COSY, TOCSY, ROESY, H-detected 1H,13C HSQC and HMBC experiments. It was found that the polysaccharide has a branched tetrasaccharide repeating unit containing 2-acetamido-3-O-[(S)-1-carboxyethyl]-2-deoxy-D-glucose (D-GlcNAc3Slac, N-acetylisomuramic acid) with the following structure: [

STRUCTURE

SEE TEXT]. Serological studies with O-antisera showed antigenic relationships between P. alcalifaciens O32 and O29 as well as several other Providencia and Proteus strains sharing putative epitopes on the O-polysaccharides.

摘要

未标记

通过温和酸降解产碱普罗威登斯菌O32的脂多糖获得O-多糖，并通过糖和甲基化分析、三氟甲磺酸溶剂解、1H和13C NMR光谱（包括二维1H,1H COSY、TOCSY、ROESY、H检测1H,13C HSQC和HMBC实验）进行研究。发现该多糖具有一个分支四糖重复单元，包含2-乙酰氨基-3-O-[(S)-1-羧乙基]-2-脱氧-D-葡萄糖（D-GlcNAc3Slac，N-乙酰异Muramic酸），其结构如下：[